LOCUS       EY195484                 469 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22h03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195484
VERSION     EY195484.1  GI:159497948
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 659
            PolyA tail found inside read...SR being truncated to 620
            Potential full insert read...vec-to-vec length: 541
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca acagttgtcg ctgcttctgc gaaagccaac tcgacttcgg cgtacatgaa
       61 gactgctgtc gatgtcgagg cctgtgctga ggagactaag aacaatgaca tgtactgcaa
      121 gggttgtccg aactgtaaca tgtcttcgga ggccatccgt aatttcgcta cgctcatggg
      181 taacatggcc gccaaattca aagaatttgc tgattcgttg ggcgggcccg aatgagagga
      241 cgagcaagaa ttatgattta tgaattatct ctatgctgat gaagaggctt ccttgttcct
      301 tcccagtccg aaccaattcc accattcatt catttttcac aatcatcctg tggcttaatt
      361 catttcatca agaaaacttt tccaaatcca aatgtttaca ccttcaaaat aaaggaatat
      421 acgcgtttat gcacacaaac atttctgtat ctgaattaaa tgtggtcca
//