LOCUS       EY195483                 495 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22h02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195483
VERSION     EY195483.1  GI:159497947
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 688
            PolyA tail found inside read...SR being truncated to 646
            Potential full insert read...vec-to-vec length: 567
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg gagaagggtt ccacgtgaac agcagttgaa tgtgggtcag ccgaacctaa
       61 ggcaccggct aacgccgtca cacactacgg ggaagactgg tgctcgcact tgtccacccg
      121 gttgccgaaa gggaagtggg ttaacattcc cacgccaggc tggggagatg gccgttcgca
      181 agagcgacta cgcgtggcga cacaaatgaa ctcgcagacg ttgtgcggag tccgggccag
      241 agttctcttt tctggtttag ggcgccagtc cctggaattg gctcagcctg agatagggac
      301 ggcgcgcccg taaagcaccg cggttcctgt ggtgtcccaa gcgctgcgca tgacccttga
      361 aaatgcgagg gaagcgttgt gaatctcccg cctgctcgta ccgatatccg cagcaggtct
      421 ccaaggtgaa cagcctctag tcgatagaat aatgtaggta agggaagtcg gcaaattaga
      481 ttcgtaactt cggga
//