LOCUS       EY195482                 373 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22h01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195482
VERSION     EY195482.1  GI:159497946
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 772 was extended to value 773 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 775
            PolyA tail found inside read...SR being truncated to 526
            Potential full insert read...vec-to-vec length: 445
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcca caaggagcag tggctgttcg cgaagggcat cttggcacgg ctgaaaaatg
       61 cgaaaaacga cgaaaaggga atgtgcgccg atgactcctt cagactggag ctgcgccagc
      121 atttgggcat ggcggaggga gagacagtgg cccaacggaa gcagaggctc caacaacagg
      181 aaggagcgtc gaaagggctg ggcgcctttt tgggggaact gttcgccgga aagacgggcg
      241 tcgccagact ggagacgctt tgcgagagag cgctgacctt ctgcgagggc agtggccgtt
      301 gagcggacgg aaaaatgttg ccaccaaaaa ctgtccattt ttgaaaattc agaggtacat
      361 ttccggagac tta
//