LOCUS EY195479 360 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab22g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195479 VERSION EY195479.1 GI:159497924 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 360) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 554 PolyA tail found inside read...SR being truncated to 512 Potential full insert read...vec-to-vec length: 432 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..360 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg ttgaatcaga gcaactaagc tgagcgcacg gtcttgcacc ggcgccgtat 61 cattcgagct tctgccctat caactttcga cggtagtgtc tctggctacc gtggtggtga 121 cgggtaacgg aggatcaggg ttcgactccg gagaaggggc ctgagaaatg gccactatgt 181 ctaaggatag cagcaggcgc gcaaattacc cactctcagc atgaggaggt agtgacgaga 241 aataacgagg ccgttctcat acgaggccgg ccattggaat ggaagaggtt taaaaccccc 301 attgagtatc tacgagaggg caagtctggt gccagcagcc gcggtaattc cagctctcga //