LOCUS       EY195474                 306 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496951.1| Putative nuclear protein, with 2 coiled
            coil-4 domains, of eukaryotic origin [Caenorhabditis elegans]
            emb|CAB76745.1| Hypothetical protein W03H9.4 [Caenorhabditis
            elegans] emb|CAB76738.1| Hypothetical protein W03H9.4
            [Caenorhabditi, mRNA sequence.
ACCESSION   EY195474
VERSION     EY195474.1  GI:159497919
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 809 was extended to value 810 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 501
            PolyA tail found inside read...SR being truncated to 458
            Potential full insert read...vec-to-vec length: 378
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tcaacatttt cttccccgat ctgctcgatc caacgcaaac gccgaacttc
       61 acgcttgtga agtgcgacga ccccgacttt gccattctcc gcttcagggc tggtcccccg
      121 tacgaagaca ttgcattcaa gattgtgaac cgggaatggg aggtgaatca caagcacggc
      181 tacaagtgcc agttccagaa cggcatcttc caactctggt ttttcttcaa ccgataccga
      241 tatcgccgat aaaacctgca ccacctgtta gcctattacg tatatttcgg ataaaaatgc
      301 aacaga
//