LOCUS       EY195471                 487 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22f11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195471
VERSION     EY195471.1  GI:159497902
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 681
            PolyA tail found inside read...SR being truncated to 638
            Potential full insert read...vec-to-vec length: 559
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg gaacgtgagc tgggtttaga ccgtcgtgag acaggttagt tttaccctac
       61 taaatgcccg ttgctatgat agtaatcctg cttagtacga gaggaaccgc aggttcagac
      121 atttggttca tgtgcttggt cgatcggcca atggtgcgaa gctaccatct gagggattat
      181 gactgaacgc ctctaagtca gaatcccgcc taggcacgat agcaataccg ttgctccccg
      241 gtgtcgggag gcccagtgta tgtgttccgc aggaatacag cccaatgccc cagcatctgg
      301 cccggcgcgc tcgcttaact gtgggcgcgt tgccaaagcc gtagcactcg tgtgccatgg
      361 tggaacgtcg gggcgcaaga tcacttgtat acgacttgac tactggtcag ggggttgtta
      421 gcagtagagc agcctttatg ctgcgatctg ttgagactca gcccttgatc ggatgatttg
      481 ttttaga
//