LOCUS       EY195467                 491 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_499752.1| Thiolases [Caenorhabditis elegans]
            pir|T22531 hypothetical protein F53A2.7 - Caenorhabditis elegans
            emb|CAB04455.1| Hypothetical protein F53A2.7 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY195467
VERSION     EY195467.1  GI:159497898
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 685
            PolyA tail found inside read...SR being truncated to 643
            Potential full insert read...vec-to-vec length: 563
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg gggaagtacc acctgacacc gatgctgcgt gttgttggct ggcagcaagt
       61 tggctgccac cccaccataa tgggcatcgg gccggtggag gccatccgca aactgtgcgc
      121 caaaacgggc atttcgcttg acaaagtgga cctggtcgag gtcaacgaag cctttgcacc
      181 gcaagtgctt gctgtgcaga aggaacttgg cattgactcc gatcgcctga atgtgaacgg
      241 cggcgccatt gcccttggcc atccgctcgc ggccagcggc actcgcataa ctgctcatct
      301 tgcgcacgaa ctcaagcgtc gaaacgccaa gtttggaatt ggctcggctt gcataggggg
      361 aggtcagggc atcgcgtgta tgttcgagat gatcaacgag tgatgagcag atgcaatgga
      421 agtgaatcta tatgcacctt attttcgaat ctatgaccta tttatacgga agacgataaa
      481 cttactcacg a
//