LOCUS       EY195464                 324 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195464
VERSION     EY195464.1  GI:159497895
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 515
            PolyA tail found inside read...SR being truncated to 474
            Potential full insert read...vec-to-vec length: 396
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtga gagccgagaa gtgtttgacg tggactttgc aaccgaattt ggggatgaag
       61 ccgattcgcg tgtaaaacgg aaagctctga agcgaagaat ggtagatgaa gagcaagaag
      121 aggacgatga tggacttgcg gggagtgata ttagtgaaaa cgaagctgat gaggatgagg
      181 atgaatttgg agcaggacaa agtagatttg gggctgatgt tgaagacgaa gaggataatg
      241 acatgatgga agaatgtgac aacgatgatg aaaatgagga ggaggaggag gaattggaat
      301 ttgactaata aaccgtccag ttta
//