LOCUS       EY195461                 437 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195461
VERSION     EY195461.1  GI:159497881
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 629
            PolyA tail found inside read...SR being truncated to 588
            Potential full insert read...vec-to-vec length: 509
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggta cgcaaaacag aatccattgt cgtaaatgaa tttctgagtt tgctcaccaa
       61 acatggcgtc gaagtgtcag aggccaacaa aacgaggatc cgacggatga tggacgagca
      121 gcaggcacta gtcaggtaga atagcaatgg cttttggatg aagaaagcgc tcttttgcgc
      181 caagaagacg tggattttga gcgcaataag agtcgaaacc agcagcaggg tggtctagga
      241 aaacccagga ttcccttgcc cgattatgag atcgaacgaa cactcggaaa tttgatgaag
      301 gaggaacact tttctgctga tcagcaggat gaggaggacc ccttcttgga gaagaggttg
      361 agaaagtcgg acagtctcta ccgataatca ttcaattgtt aagttttacc tggagccata
      421 cataaattgg tcattca
//