LOCUS       EY195456                 200 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195456
VERSION     EY195456.1  GI:159497876
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 636 was extended to value 636 (,)
            putative full insert read: vector-to-vector length is 343 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 490
            PolyA tail found inside read...SR being truncated to 351
            Potential full insert read...vec-to-vec length: 272
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tcggattttg agctctcttc cagtgactac tcgcaatgaa tttgatctaa
       61 ttctcatctc tgtcccctat tgcctaatcc gacccacaac catccatctc atatcagcac
      121 taggtgaacc tgatgatata catcttgtcg attattgact gactgaatca ctttacttct
      181 gggtataaaa tattaaatta
//