LOCUS EY195454 171 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab22e06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195454 VERSION EY195454.1 GI:159497874 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 171) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 801 was extended to value 801 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 364 PolyA tail found inside read...SR being truncated to 322 Potential full insert read...vec-to-vec length: 243 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..171 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag tttgatgaaa acaaattgta atcatccgca gctaacgcac ggaatgcgag 61 aacatactgt tatcacgtac accaaagtgc tctgaaaact cgaccgtcga attttaaagt 121 gttggctttg tgataaagaa gatttaaaaa agaataaaaa cgaatgggcg a //