LOCUS       EY195451                 434 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22e03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195451
VERSION     EY195451.1  GI:159497871
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 853 was extended to value 854 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 626
            PolyA tail found inside read...SR being truncated to 585
            Potential full insert read...vec-to-vec length: 506
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa agccgggcgc ctatatttca cgcgagtggc gcatcgatat ccacacctag
       61 aattgaggag gctgaggact gtgtgttcag gcaggtatga agaagcgggg agtaagggcc
      121 accctcccca tcagcctcat tccctctctc agctattggt actcttttca cccccattca
      181 ttcaccgttt tgccattttt tcggattgga ctgacggata ttcacataca agttgttttt
      241 gccgcccaag ctaaataatc ttgaccccaa tactttgagt cgggacaggc ttgtgttatc
      301 gtcgttaacg tgtttactta agtaataggg tactcattat tactcagata cactacttcc
      361 acgtgattgt tttgtgagga taaattattt ggttgggttt gtattatatt ttggccaata
      421 aatattcgca tgga
//