LOCUS       EY195450                 485 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741145.1| Myosin light chain MLC-3 (17.1 kD)
            (mlc-3) [Caenorhabditis elegans] sp|P53014|MLE_CAEEL Myosin,
            essential light chain (Myosin light chain alkali) pir|T16012
            hypothetical protein F09F7.2 - Caenorhabditis elegans
            gb|AAA28112.1| alk, mRNA sequence.
ACCESSION   EY195450
VERSION     EY195450.1  GI:159497848
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 680
            PolyA tail found inside read...SR being truncated to 637
            Potential full insert read...vec-to-vec length: 557
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tggcgatgtc gttcgtgcat cgggattgaa acccaccaac gcaatggtgc
       61 acaaggcgac aggacaagag tataagcgca agggtgaaaa gcgaatcacc ttcgaagaat
      121 ggttgcccat ctacgaacaa ctttccaagg aaaaggacgt ggggacctac gctgattttt
      181 tggaggggct gaaagtgttc gacaaagacg aaagtggtaa gattatggcg gcggaactca
      241 ggcacattct actggctctt ggggaacgac tgagtgcagt agaggtcgac gaactgctaa
      301 aaggagttga agacggcgat ggcatggtca actacgaaca gttcatcaag aaagtgctag
      361 ctggaccgta cccttcggat gactagaaat gaatattgct cgagcccgag gaaattgcgg
      421 aatttcaaac gtattccatc ttaaatttcg gatttgttga aaccgagata aaagtggaat
      481 tcaca
//