LOCUS       EY195445                 423 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195445
VERSION     EY195445.1  GI:159497843
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 623
            Potential full insert read...vec-to-vec length: 422
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac acacacattt gtttgtgcgc cgccaactct cccgagagga gccaacccgc
       61 atcccactga tactatttct gtatttctgc tcactcgctc aacgccaaac agcgacacaa
      121 tgctgggtct gcagttcgcc tacttggagc ctgccgccgt cagcgcccaa tcggaggagc
      181 agctgctgca agagtgcgcc gaagtgaagg acgggttcgg gcgtgcggag cggcagcagc
      241 agccgacggc agaagcccaa gcgccgcagc agcagcagca gcgcttcatc gcacgagtgc
      301 tccagcacat tggcagtggc ggccgaacag ccgaaaatgg cgcagacagc aacagcacac
      361 acaattactg ctctttgcca aacgatgatg atccttgaaa cgttgagaca gtaaaaactt
      421 ctg
//