LOCUS       EY195442                 394 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195442
VERSION     EY195442.1  GI:159497840
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 590
            PolyA tail found inside read...SR being truncated to 546
            Potential full insert read...vec-to-vec length: 466
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt cagttctgag agcagttcat cgactgacac atttgacgaa gaggagataa
       61 agcagcagct gctgaaccgg ttgaagaacg aacagcgcag agagatcgcg aagaagattc
      121 aaatgcgcat accaaaagaa ctgccggcgt tcaaacagca ggcgctgaag cgacaaaagg
      181 ccgaggtgtt gcgaaacctc ggccacgaag gttgttctcc ggccaaaaag aagtttgaac
      241 gcttcccggt aacgatgatg gcacaacaag ggacggtaac ggggaaacac gatgtcgagg
      301 aggcgcggat ggaagctatt tctgattccg aagacattat aattgaagaa caacagcccc
      361 agcccaaaaa gggtgctgca cttcagcaac agca
//