LOCUS       EY195419                 295 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_660071.1| unknown [Rhizobium etli] gb|AAM55072.1|
            unknown [Rhizobium etli], mRNA sequence.
ACCESSION   EY195419
VERSION     EY195419.1  GI:159496755
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 491
            PolyA tail found inside read...SR being truncated to 446
            Potential full insert read...vec-to-vec length: 367
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gaagaatggg aaaagataaa aaacagctta cctgggaagg aaggagatcg
       61 tgggaggagt gcaaaagata atagaagatt tatagcagca gtaatgtgga actggagcac
      121 catggagagc attgcctata gagtatggaa aatggtcaag aaaggttcat aagatgggca
      181 aaatcaggtg tttggcagat gattttcaat acattggctg ttgatgaaga tacagaatgg
      241 ttaatgatag actcaacaat aataagagcg catcagcatg cagctggtgc aagga
//