LOCUS       EY195417                 462 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22a12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195417
VERSION     EY195417.1  GI:159496753
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 655
            PolyA tail found inside read...SR being truncated to 613
            Potential full insert read...vec-to-vec length: 534
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac cgccatcagg acatcactgt ggtcatcttt tcgggtgttg tgctcgcggt
       61 gtccatcgct cttttctatg cgggcagtta catccaatcg gaacgtgcgc aggccgatct
      121 gatacaatat tcgcgtctac aagtcccccc tcgcggacct cgctcccgcc tattgtcttc
      181 ctcgactctg gattcgtccg cctatctcgt caattccacg cccacatcgt caacccacgg
      241 atgaagtatt tttgaattct tcgtgcagcc cggttatact ccccacggca gaaaggcttc
      301 gaaatcggga ttcactcgaa tttgtttcat tacacctaca ctttctcttt tgcaacccat
      361 ttgttctttt gcactacttg aacccatccg atgttttaaa aaacccttta tctggttcct
      421 tccatttgcc tgtgctcata tttttgataa aacgatttat ga
//