LOCUS       EY195416                 365 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195416
VERSION     EY195416.1  GI:159496752
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 775 was extended to value 775 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 558
            PolyA tail found inside read...SR being truncated to 517
            Potential full insert read...vec-to-vec length: 437
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca agaaccgagt gtgtccagtg gtagcgaaga aatgggtgct ccagaggccg
       61 aagaggcttc ggcacgcaag aagcgtcaat tcactgttga caacagagtg aagcgtcaat
      121 tcacggttga caccagggtg aagcgtcaac tcaagcttgg aaacagagtg aaacgacaat
      181 tcaagcttga caacagagtg aaacgacaat tcgaacttga acgcaagaaa cgtcaattta
      241 cgttgggcaa caacgtttaa aatagaaaac gatggaagcc gatgagatcc atagccaaat
      301 aaagtataag acagagagct ttgatgataa agtacaattg cttttggata aatgttaacg
      361 tgtca
//