LOCUS       EY195410                 474 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab22a04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|T29005 hypothetical protein ZK328.3 - Caenorhabditis
            elegans, mRNA sequence.
ACCESSION   EY195410
VERSION     EY195410.1  GI:159496714
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 667
            PolyA tail found inside read...SR being truncated to 625
            Potential full insert read...vec-to-vec length: 546
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac tccaagacgt gtgagaactt tatttccatg ttccgctgga catgaaacgg
       61 agctgaattt cgaagcaaac cagattataa cgaatgtcta tccctcaaaa gaagacggat
      121 ggcttgtcgg tacattgaat ggaaggactg gtctaatacc gagcaactac gttacattca
      181 tttaaattct taaatctact actgcgttcc agaatattct tcaatgttgt gctgacattt
      241 gcaacttttc gcttgtttca tcgtctcatt ataatccgct agagtcaaaa tcaaaaggct
      301 ttttgcatca ccatcgtttt gctttttggt agctcaattc tataataaca tctgatcaaa
      361 acaacaatga tattacaaaa gcaaaattat tagattttta tggtcttata ttaatattat
      421 aaatcgcttt catttaatct gaaatactca tagacggtaa ataaacaaat atga
//