LOCUS       EY195401                 256 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21h07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195401
VERSION     EY195401.1  GI:159496674
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 825 was extended to value 826 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 450
            PolyA tail found inside read...SR being truncated to 408
            Potential full insert read...vec-to-vec length: 328
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gggcgctgtg ccaccaggtt tgcaagggcc agtgcgcgga atggggggac
       61 ccggaatggg agggcctatg cagccgggtt acatgccgcc gggcatggca ggtggaatgc
      121 cgccgcgccc tcactgaaag atcttggatg gatttgttct caccaatttg acgtattttg
      181 tttatcgatg gtccagctga tctgatgagt actggtttca tttgtaactg gccgaatata
      241 aattattttt cgatga
//