LOCUS       EY195397                 520 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21h03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_724578.1| boca CG30498-PA [Drosophila
            melanogaster] gb|AAF59229.2| CG30498-PA [Drosophila melanogaster],
            mRNA sequence.
ACCESSION   EY195397
VERSION     EY195397.1  GI:159496670
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 819 was extended to value 820 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 707
            PolyA tail found inside read...SR being truncated to 671
            Potential full insert read...vec-to-vec length: 592
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa caatgggaag agaatgacca agatgaattg tctcgagatg aattgcctaa
       61 tcagaaaaag ccaccgaaca ttgatcttga ttctctccga aaaaaggcta aatcgccgga
      121 tgaactgatc aaattaacta aacgagggca atcagttatg atgtttgtat cagtgaaaaa
      181 cccagctctc gattcatcta ccaagacacc aagagatttt actgatagga tgtcttcgtt
      241 ctggcaaact aattttttca acaatcacat tgacgcacaa gttttcattg ttgacgatga
      301 tcgagtcttg ttcctgttcc gagaaggttc tcaagcttgg gatgctaaag atttcttgat
      361 taaacaacca gaatgcaagg aagttgtatt ggagggcaag acattcccag gaaccgctca
      421 aattcccgag aaagaacttt aatctcaaaa cacaattcaa atataccaag ttcaggcctg
      481 gttcatacat tcatttaatt ttgttaagaa atgaattcta
//