LOCUS EY195388 251 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_499055.1| Putative cytoplasmic protein of eukaryotic origin (18.5 kD) [Caenorhabditis elegans] sp|Q03598|YLF6_CAEEL Hypothetical protein C40H1.6 in chromosome III pir|S28301 hypothetical protein C40H1.6 - Caenorhabditis elegans emb|CAA7955, mRNA sequence. ACCESSION EY195388 VERSION EY195388.1 GI:159496632 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 251) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 444 PolyA tail found inside read...SR being truncated to 401 Potential full insert read...vec-to-vec length: 323 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..251 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgc ggcggcaaga tttgcctgag tgaccacttc aagccgctgt gggccagaaa 61 tgtgcccaag tttggcattg cacacgcatt ctcacttggt ctcggtccgt ggctggcggt 121 cgagattccc gatttgattg agcgcggtgc tataacagca aagtcataat ttattcggct 181 ggagattgag gttttgggct gtgataccaa tttggacttg ggggttggaa ggataaatat 241 tgatgttaac a //