LOCUS EY195381 230 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21f09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P41042|RS4_DROME 40S ribosomal protein S4 pir|JN0871 ribosomal protein S4 - fruit fly (Drosophila melanogaster) dbj|BAA03786.1| ribosomal protein S4 [Drosophila melanogaster], mRNA sequence. ACCESSION EY195381 VERSION EY195381.1 GI:159496594 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 230) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 810 was extended to value 810 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 424 PolyA tail found inside read...SR being truncated to 382 Potential full insert read...vec-to-vec length: 302 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..230 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtat cggcaagggc gcaaccccgt tgatcagtct gccggcggac aagggcatca 61 agctctcgat tgcagaggag cgcgacaagc gactggccca gaaagtgaaa gccagccact 121 agacgagtgg tgatggatga cttggtgggt acacaaaaaa atttgttgat ttgttatctt 181 gaacgaatac ttgtgttcac attggctgat aaacacgatt tctgttaaca //