LOCUS EY195379 166 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491061.1| Ribosomal Protein, Large subunit RPL-1 (24.1 kD) (rpl-1) [Caenorhabditis elegans] gb|AAF36008.1| Hypothetical protein Y71F9AL.13a [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195379 VERSION EY195379.1 GI:159496592 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 166) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 827 was extended to value 827 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 392 PolyA tail found inside read...SR being truncated to 353 Potential full insert read...vec-to-vec length: 165 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..166 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ctggcagaat gtgcgctctc tgaacatcag gtccaccatg gggaaggcgc 61 agcgcctcta ctgattgggc gccatgcgat cgggagagtc cattcaattt gttgtttttt 121 tatttttcca aaccattgtt gaatttggag gagtaaaatg ttgatt //