LOCUS       EY195373                 516 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195373
VERSION     EY195373.1  GI:159496556
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 804 was extended to value 805 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 703
            PolyA tail found inside read...SR being truncated to 667
            Potential full insert read...vec-to-vec length: 588
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat caaggaggaa ttcgcatccg gtgccgggag acgtcgcttc tttgtggaac
       61 tgccgcccaa tgcgttgaac cattgggaag agcgatggct ggaaaatggg ttgggcgagc
      121 ggcttgccat ctgccagacg tgcattttgc gctgtcccat tggggaagag cacaagcagt
      181 tcgaagactt ctacgtgcac ctgcggcaaa agcagggacc gctgatccgt gtggacattt
      241 gctcgaccgg ctggctcaaa agcccgatgg tgacgcgaag aaacgggaag tgctgattga
      301 tgagaagtaa atctgctcga agtgcagttt gtacgcaaaa ttcccgttga cctttatagt
      361 catcgtagta attgtttcgg tagatgtgca attgtacata gtacatatat gattattgat
      421 tttattttta cagatgtgat tattaattaa attaaagtaa atatgatttg tgaaattaat
      481 tttaaatttt gtaaaaataa ttatggttaa cgcaca
//