LOCUS EY195365 376 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195365 VERSION EY195365.1 GI:159498511 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 376) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 570 PolyA tail found inside read...SR being truncated to 527 Potential full insert read...vec-to-vec length: 448 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..376 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtgc tgcaggcatg tcgcaccgtc ccgccactca tcatcgtccc ttccctgtcc 61 cgccccggca cacccttcct gtccagacat tcaactgcgg tgtggtcgaa gtctctccca 121 agtcaatacc cgatgctccg ccgccatacg aggagtttgt gcgtgttcca ccaccaccgc 181 cacaaagggc accgcccatt ctgacgcggg aagaggatga ggagttgcag aacagactga 241 actcggagag agagagggaa ctgagcgact ggtgacattt ggttttgtcg agtgctgcag 301 cttcgcacca tttcccttta tatacgggac tttcttcatt tcttttgttc ctgacttaac 361 aacaattaat agacca //