LOCUS       EY195355                 257 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195355
VERSION     EY195355.1  GI:159498501
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 823 was extended to value 823 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 450
            PolyA tail found inside read...SR being truncated to 409
            Potential full insert read...vec-to-vec length: 329
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcag cgctgaatct tggtgcctct ggcgccaaga ggtgtagcgt acagactgcg
       61 agtttgggct ggcgcgtcag ttcaagtctc ccttgacagg ggtccctcag aaggtgcaag
      121 gcctgtccaa cgggtgcaga cagtccattt tcgcgtgtct tggagtcggg ttgtttggga
      181 tcgcagccca gagaaggtgg taaacttcat ctaagactga atatgactac gagaccgata
      241 gcgaacaagt actgtga
//