LOCUS       EY195354                 435 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195354
VERSION     EY195354.1  GI:159498500
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 847 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 627
            PolyA tail found inside read...SR being truncated to 585
            Potential full insert read...vec-to-vec length: 507
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag aagaagaaaa tggaagacag acaaaaggcc gaagccgaaa acgtaaaact
       61 ggatgagaag aagaaaatgg aagagaaaca aaaggccgaa gccgaaaaag taaaactgga
      121 tgagaagaag aaaatggaag agaaacaaaa ggccgaagcc gaaaaaggaa aactggatga
      181 gaataaacaa aaggccgaag ccgaaaaagt aaaattggat gagaagaaga aaatggaaga
      241 caaacaaaag gccgaagccg aaaaagtaaa attggatgag aagaagaagt tggaagacaa
      301 acagaaggct gaagccgaaa aagttaaatt ggatgagaat aaacaaaagg ccgaagcaga
      361 acaagtaaaa ttggatgaga agaagaaaat ggaagacaaa caaaaggctg aagccgaaaa
      421 agttaaattg gatga
//