LOCUS EY195349 350 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21c09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_490842.1| SUMO (small ubiquitin-like modifier) SMO-1 (10.2 kD) (smo-1) [Caenorhabditis elegans] sp|P55853|SMT3_CAEEL Ubiquitin-like protein SMT3 pir|JC5582 SMT3 protein - Caenorhabditis elegans emb|CAA67914.1| ubiquitin-like protein [Caeno, mRNA sequence. ACCESSION EY195349 VERSION EY195349.1 GI:159498495 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 350) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 822 was extended to value 823 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 543 PolyA tail found inside read...SR being truncated to 500 Potential full insert read...vec-to-vec length: 422 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..350 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcga gtcaagtacg gtactagtat gggaaagctg aagaaagcct atgcagaacg 61 aaccggagtg caggtgagtt cgcttcgctt tctctttgat ggtcgtcgcg tcaacgatga 121 ggacactcct aagtccctgg agatggagga agacgatgtc attgaggtct accaagaaca 181 actcggaggc aatgcttatc cgaagaactg aggcgagatc tatgaacctc tgaaggccac 241 caccagttcc ggaagattcc ttgacacctt attttttgca atcgagcgca tctcgcatca 301 tttcttttct gttgcttaat ttatgctcta ctaaaaaatt tctcatccga //