LOCUS EY195343 191 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab21c01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195343 VERSION EY195343.1 GI:159498489 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 191) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 815 was extended to value 815 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 431 PolyA tail found inside read...SR being truncated to 342 Potential full insert read...vec-to-vec length: 263 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..191 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtt ccaaaggggt cgccagaaat gcgaagccgg tctcccgctg atcatcgctg 61 cgcactgggg cagcagaatg agcttggcgc aaatgcagaa tttctacggg tcaatcaaat 121 cagtggactg ttgacaataa tacatattgt ttattgtttt ttctgatgta caaaatgtgc 181 aattttaaac a //