LOCUS       EY195333                 421 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab21b02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195333
VERSION     EY195333.1  GI:159498479
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 827 was extended to value 828 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 612
            PolyA tail found inside read...SR being truncated to 571
            Potential full insert read...vec-to-vec length: 493
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag gactgacggt ggagccaaag ccgaatgcac aacacaagga tgacgaatga
       61 ggaaagaaaa taagagaaaa tgttcaattc cttttagtga tcgagtgctg ttccatttct
      121 actcccccac tctcacgttg tctcccgttt cgatcattta tgcgcgggga cgcgcattcg
      181 gggagccacg ggaatgttat gggaacaaag tccatgagta ggagcaaacg tcaatggggg
      241 ccatggctag ggccaagccg ttaagccttc cataaatcat tgtggcccac caatgctctg
      301 gggagaaggc atcttcttta aagcccttcc cttttctatt cttggggctc attctttata
      361 tcgcccaacc atcagccgat gttacggagt gacgaaccag cagaataaag gattgaaccc
      421 a
//