LOCUS EY195300 563 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab19g01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195300 VERSION EY195300.1 GI:159498446 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 563) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 800 was extended to value 800 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 802 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..563 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgc cttcccttcg gtcgctttat ccacagccgc caaaaccaca aatcaaagtg 61 acgcgtggaa aaacggaacg accaagcgaa tggacggatc tcgaagagcg tcttgcccaa 121 ttaagtaggc caatagaatc gacagttgaa gcagaaaagg aaaagcaaaa gagccccaaa 181 aaaccggttg ccagcattag cgaactaaaa gaacgccttg cagccctacg cggtcttcca 241 gtggatatta ttcgcaagcc tcgactgctt gttatgaatg aagatgacga cgacgatgct 301 gctttgacgg atgatgcaat ggagttactc agacaggtcg agcttgggtt gaatctggac 361 agccgtgttg cggtcggcat caacaacacg tctggtccaa gcaatggcag gttgcaagta 421 cgaagcgagg gaatggagga cgcgcaatcg accaacagca tatcgtctac aatgttgggg 481 caagttgtaa atgaggcgct agaagcgccc tcggctggta gcagcacatc cacactgtct 541 cgggctgcca gcgaacaaat cta //