LOCUS EY195074 208 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab17a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195074 VERSION EY195074.1 GI:159498202 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 208) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 768 was extended to value 773 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 402 PolyA tail found inside read...SR being truncated to 359 Potential full insert read...vec-to-vec length: 280 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..208 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggga gaaacaagcc gagaaggtgg cgcgccgtca ctcctctgcc tcgcactcaa 61 agtccgagtc gaaggtgtcc gaatcggaac ccagggtcgg cggtaagaag tgagcgccag 121 gacgacacca agttttggac aaattttgca agttgtttcg tggaatattt tgttgatgtg 181 ttaaaaaatg gttaaacttg tttcatga //