LOCUS EY195073 244 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab15h12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195073 VERSION EY195073.1 GI:159498201 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 244) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 459 (,) original QR value of 759 was extended to value 759 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 607 PolyA tail found inside read...SR being truncated to 395 Potential full insert read...vec-to-vec length: 316 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..244 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac gatgtggaaa tggaagacaa aggcgaggaa gaaagtaact gcgattcgga 61 ttacgacccg gcgaggaata agggggtcgg aaagaccagg aaggctcaaa aagtaaggta 121 ccctagcaac gcagtttaac gtgttcttct cgatgggctt gattgatttt gatctattga 181 ctttaaaatc cattacgacc aatccgttta ctctaacagc caagataaag atacatatgt 241 atta //