LOCUS EY195072 244 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab15h11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195072 VERSION EY195072.1 GI:159498200 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 244) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 289 (,) original QR value of 598 was extended to value 599 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 439 PolyA tail found inside read...SR being truncated to 396 Potential full insert read...vec-to-vec length: 316 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..244 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat cagcgcaatt gccaacaatc cggctttggc ggccaaggac aaggccgcac 61 agattcaggc catcgtggag ggactgccgc agaatgtgcg caacgagatg gagaaagcca 121 tgggcattgg ggggcactga acaacgagca atcgagtgga aacgaaaaac accggttgat 181 tcaatgacac tcgaagctga attgaatggc tttgcacaaa tcacagtaat aaacagtatt 241 tcca //