LOCUS EY195066 454 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab15g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_496764.1| Iron-containing alcohol dehydrogenase (50.5 kD) [Caenorhabditis elegans] pir|T26686 hypothetical protein Y38F1A.6 - Caenorhabditis elegans emb|CAA21631.2| Hypothetical protein Y38F1A.6 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY195066 VERSION EY195066.1 GI:159498194 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 454) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 829 was extended to value 829 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 834 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..454 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggccc aacaaagcat gcagaaggcg attgactttg ctcgcgcccg acccttcgat 61 tgcttcctgg cggtgggcgg cggttcagtg atcgacacag ccaaagcggc tgcactatac 121 gcgagcaatc cagaggcggg atttttcgac tttgtgcagc agccattcgg aaagccactg 181 atccctaaaa attcgatgct gcctctaatt gcgataccta caactgcggg aaccggcagc 241 gagaccacag gtgtttcaat aatcgacatt cccgagaagc agtgcaagac cggcattcga 301 ctacgttgca tcaaaccgct gctggcagtg attgatccac tgaatgtgat gtcaatgcct 361 cgaaatgtgg ctatctactc gggatttgat gtgctctgcc atgcgttgga gagttacaca 421 gcgaaaccat ataaccaggc gcccaccgcg cccc //