LOCUS       EY195057                 354 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15f12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195057
VERSION     EY195057.1  GI:159498185
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 160
            original value before phred recall for SR was 559
            PolyA tail found inside read...SR being truncated to 521
            Potential full insert read...vec-to-vec length: 431
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa caactgacca accgtgccac attgctcgct gttaatgggt gcacactaca
       61 gctggtcgaa acgacaccgt cggggattcg gccgatgcgc gttgcggaaa atgaagagga
      121 attggaggca ttgcaggact gggagggaca ctggcgcaag ttccggcgat ggctgatcgg
      181 cctcaactgg aaagcactgc tccagtgatc tgcaaaccaa tttatcttct atttgtgaat
      241 agataaactt tctgagcgag agagaagtaa ccaagtgata tctgatattc aactgattgt
      301 ataataaata tcgtggaaaa tttcaactta agaaacaaaa caaagaaaac ttca
//