LOCUS EY195053 431 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab15f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY195053 VERSION EY195053.1 GI:159497117 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 431) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 831 was extended to value 831 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 624 PolyA tail found inside read...SR being truncated to 582 Potential full insert read...vec-to-vec length: 503 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..431 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtt ggaggtgctg agcactgaga tcgtgggtcc gctgcgcgag ttggacgcgc 61 acgcggtaga gctggagctg ctgcgctgca tctgcttctt cagcacaggg ccacagctga 121 gtgcggacgc ccatcgagta gtggcctcgg tgcaaagcag ttacctgaag gcgctcagcg 181 agttcgcgct gaagcagttc gcgacgggcg aacagcacaa gcacccggac agcgctgccg 241 gacacactct gctggatgcc gtgttccgcc tcaaccgcct catcaacgtc ctctccgcgg 301 tcgagaaaat ttcgtatcag tacgactgac ggaatgcggc ggaggaagct ggcgccgttt 361 aattccgaaa catccccaca cttgccgaac tgtacacaaa cattttatgc gataaagatg 421 tcgacatccg a //