LOCUS EY195049 555 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aab15f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_142081.2| similar to ubiquitin conjugating enzyme (E217kB) - rat [Mus musculus], mRNA sequence. ACCESSION EY195049 VERSION EY195049.1 GI:159497113 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 555) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 825 was extended to value 825 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 835 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..555 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc cgcccgacac gccgttcgag ggcggcgttt tcagcgtcca aatgcgattc 61 ccgaacaact atccgcaccg gtacccaagg ctcaacttca cgacgcccat ttaccacccg 121 aacgtggacc ttgccactgg gctggtggcg atggacctgc tctcgtggga caaatggacc 181 gcaccgacgg acattgcgca gattctgttg gctctccacc agcttctggc cgaaccggac 241 agcgagaggg ccaatgagct ggacatctcc ggactgtttg agaccgaccg ggagcagttc 301 aacgcgatgg ccaccgagtg gacggtggca catgcaatgc cagaaagcaa tgagcagtga 361 ttttgggact tgacatggat gtttggtgaa gagggcgcgg gaatattcaa tcatccattc 421 gatcagattt ttattttaat caaatttttt aatgattatt gaaaaatgta aagtttgatt 481 tttttatggc tccttcactt gcactttctt cttatatttg tgtatttatg tatcaggccc 541 atcaatggac cgacc //