LOCUS       EY195040                 354 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_504712.1| Calponin family family member (44.4 kD)
            [Caenorhabditis elegans] pir|T29521 hypothetical protein T25F10.6 -
            Caenorhabditis elegans gb|AAB04989.1| Hypothetical protein T25F10.6
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY195040
VERSION     EY195040.1  GI:159497073
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 762 was extended to value 763 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 594
            PolyA tail found inside read...SR being truncated to 505
            Potential full insert read...vec-to-vec length: 426
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga cgactcgcac gaggacctgc catcggacat ttcgcgacga cccgaagtcc
       61 ccttctggtc gggaatggag accgactttg cgaatcagca aggaatgctg gcattcggaa
      121 ctccgcgaga cgtgcgtgga gaatacgtgc gtcgaatgtg gtagaaggga gcagagcggg
      181 ggatggacag tttgtcagaa caggaaacaa aacaaacaca ctatttactc gatgcacccc
      241 aatccatttc ttccgtggac agtccccacc cacagctagt caattgccca acccaaacat
      301 tgttgaagtc agaagccgcc atttgcactt tgcaccagta aagcgaggag gaga
//