LOCUS       EY195039                 599 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15d08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_491282.1| Calponin homology (CH) domain
            containing protein family member [Caenorhabditis elegans]
            pir|T29467 hypothetical protein F28H1.2 - Caenorhabditis elegans
            gb|AAB52338.1| Calponin protein 3 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY195039
VERSION     EY195039.1  GI:159497072
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 632 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 780
            PolyA tail found inside read...SR being truncated to 750
            Potential full insert read...vec-to-vec length: 671
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggttc caggttgtgg ccaagcaggc gagcaaatac agcaaccagg agggagagat
       61 gtgcctggcc tggatcaaga aaatgagcgg cgagaatatc agcaccaacg gcgaccgtga
      121 gaacttctac aaattgctaa aagacggcac actactgtgc aagctggcca acgggattca
      181 ggcgggctcc gtcaagaaga tccagcagcc gactggcaac ttcgcctgca tggagaacat
      241 caacgctttc gtgtcgttcg cgaagagcca gggagtgccc accgaggaga ccttccagag
      301 cgtggacttg ttcgaggcac gtgacctcta ctccgtgtgc atgaccctgt tgtcgttggg
      361 ccgaattatg gagaagaagg gaaagccgaa cccattctct ggatgaagag tagaagtgag
      421 tgcagcaaaa gacggacaga gcatgtgcta tcgctcccat tcgagaactc cccgttttgc
      481 gaattttctc cccgtgtgcg accttgcaac aataccgagg cgttaactgt tttcccctct
      541 ctctcctctc aaactgtggg catttgaaaa tagcgatgcc ggaataaatg gccaattca
//