LOCUS       EY195028                 599 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15c06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_501853.1| Tetraspanin family member (tsp-12)
            [Caenorhabditis elegans] pir|T24912 hypothetical protein T14G10.6 -
            Caenorhabditis elegans emb|CAA93092.1| C. elegans TSP-12 protein
            (corresponding sequence T14G10.6) [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY195028
VERSION     EY195028.1  GI:159497001
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 617 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 765
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgc ccaaaatgca aagttggctg aacaacaact ttctttacgt cggttccacc
       61 attttcgtgg ttgctgtaat ccaatttctc ggaatatgtt ttgcacaaga tctccgcagt
      121 gacatttttg cacagcgcgc aaaatggagt cgccgttcat gagtcattcg gaatttgagt
      181 gtgatgcgaa tgcaatctac cttcatgcaa cagtgaactg gttcacagaa atgctttcaa
      241 aaatattctt ctaattatta tcgtttggaa aaattactgc aatcaaaacg gtccttcacg
      301 ctttttctaa gctcttttaa atgaatgcca tcttctctct tcacagcagt gacacaatct
      361 gtcaccattc tcaccctgaa tctgctacat aatacatcct ttgcgctctg actattattt
      421 tcatatttat attgttgttt ttgcgccttt ttgctttgac agatcccaat tgtaattcag
      481 aattcatctc taattgcatt taattgcctt cagcattttt gatcccatcg agagtttcca
      541 cccaagaagt acttttttta aaataaaaca attaaagtcc taaaaaaaaa aaaaaaaaa
//