LOCUS       EY195022                 519 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15b08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY195022
VERSION     EY195022.1  GI:159496995
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 745
            PolyA tail found inside read...SR being truncated to 670
            Potential full insert read...vec-to-vec length: 591
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac acacttctcc aatccaaatc caaaaacaag aaattcggga ttctttcttc
       61 ctttaaatgg tctccacact cttgacgcta tttcccgtgc tcgtgattgt catgatgatg
      121 gtggaggagc cggtgcaagc cagtcctagg ccggactgca cccaaatgtg ccaagattct
      181 gcttgcagcc agatatgtaa tcgcaaaaaa cgggatgaag catttgcgca gctatgcagc
      241 ggaggcgtgt gcaagacggc gagcgcgagt actggcagaa agaaaagaga gaccgcagtg
      301 tttgaagcag caaaacaaaa acgcaaacgt catttggctg ttttggcaga aaacctgaac
      361 tgatgtgaga aacaagaaat atgaaatgaa aaaatgatat taacactttt cattcacata
      421 tattttaaat cgtaaaaaaa ccaatacctt aagacgctta aataaaaatc cctcatatgg
      481 ctggtaaaga tgagctcgaa ataacaaata caaagatta
//