LOCUS       EY195021                 635 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAG15329.1|AF255955_1 beta tubulin [Chionodraco
            rastrospinosus], mRNA sequence.
ACCESSION   EY195021
VERSION     EY195021.1  GI:159496994
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 852
            COMM   possible reversed clone; protein similarity on positive
            strand
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac atgtgaactg ctcggagatg cgcttgaaca gctcctggat ggcggtggag
       61 ttgccgataa aggtggctgc cattttgacg ccgcgcggcg gaatgtcgca aaccgcggtc
      121 tttacgttgt tcggaatcca ctccacaaag taggaggagt tcttgttctg cacgttcagc
      181 atttgctcgt ccacctgcat tcaaaatcaa aaggctcatc cgccctcgga atatggcggc
      241 cacagtcaga tagcggccgt ggcgtgggtc gcacgcggcc atcatgttct gaagataaca
      301 acgattgaag ctcgggcacg gtcagtgccc gatactgctg gctgccgcgc gaagtgagcg
      361 gcgcaaagcc gggcatgaag aaatgcagca actctattct acgctcactt ggtggccagt
      421 ttccgcagat cggcgttcag ctgacccggg aagcgcaggc acgtggtaac tcccgacatg
      481 gtcatcgaga ctgcagattg aggagtcgcc gtaggtgggc gtcgtcagct tcagggcgcg
      541 gaagcagatg tcgtagagcg cctcgttgtc aatgcagaat gtctcgtcgg tgttctcgac
      601 cagctggtgc acggacagag tcgcgttgta cggct
//