LOCUS       EY195014                 578 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab15a11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_496076.1| SQuashed Vulva SQV-8, defective
            SPErmatogenesis SPE-2, Beta-1 3-glucuronyltransferase 3 (41.1 kD)
            (sqv-8) [Caenorhabditis elegans] sp|Q09363|SQV8_CAEEL Probable
            glucuronosyltransferase sqv-8 (Vulval invagination protein sqv-8)
            (Squa, mRNA sequence.
ACCESSION   EY195014
VERSION     EY195014.1  GI:159496958
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 621 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 771
            PolyA tail found inside read...SR being truncated to 729
            Potential full insert read...vec-to-vec length: 650
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatc caaaaggagt ggcgcagaga aatacagcat tgaactggat aaggctcaat
       61 gtcttgaata gaagcgaagg aatagtttat ttcggggacg atgataatgt ctacgattgg
      121 cgaatttttg gtgaaatgcg acgtataaaa cgagtcggaa tttggccagt gggcattgtt
      181 ggtggatcca ttgctgaata cccaattctc gatgaaaact attctgtcgt cgggttcaat
      241 actcactgga aatcgcaacg actctttcca gtggatatgg cggcatttgc gttgaacatt
      301 tccctcattc tcgaacaatc agaagtctac tttacatatc gcacgtctcg agggaatttg
      361 gaatctcagt ttctgcgatc tcttaatgtt tcgcgcttgg atttggagcc aatggcaaat
      421 ttgtgcacac gggttctagt ttggcacacg cgaacagaga aagcgcaact cacgaggcga
      481 gaaatggcca aatttattac aaagaacgga ctcgatggtg tggaaaagga cgccatttta
      541 tgatgactaa caaaccttaa aatatacaaa tatattga
//