LOCUS       EY194991                 537 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab14g05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741220.1| Histidine kinase-, DNA gyrase B-, and
            HSP90-like ATPase and Hsp90 protein family member [Caenorhabditis
            elegans] gb|AAK29856.2| Hypothetical protein R151.7a
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY194991
VERSION     EY194991.1  GI:159496878
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 889 was extended to value 889 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 726
            PolyA tail found inside read...SR being truncated to 687
            Potential full insert read...vec-to-vec length: 609
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga agaagggctc tcgatggagg cggaagcaga aaagacggtg accagggacg
       61 ggcagaagaa agagttcctc ggctggctga agtcgagcct cggttctgtt agggtcgcag
      121 acataaagcc aaacttccgc caatctgagc atccatttat gatcaccgtg ggagccgata
      181 tggccgcagc cagacacatg ctgaggataa ccgaagtcaa ggagatggag catctcgttt
      241 acttgcgacc aatactgcat gtgaacttcc agcaccccgt tgtctccggc attatgcggc
      301 tcaagaaaac aaatccggaa ttggcagcaa aagtgtcgga acagatttac gacaatgcgc
      361 tatgtacggc tggcttgctg aaggacacca gcaaaatggt cggcagattg aacagaattt
      421 tcgaagaatt gctgcagttg aacgcggaga agtcccaaat attgactcca tgaaaagaac
      481 ttatattgtt gaacttttca cttttgttga ctaaacaata aaaactggca gctttca
//