LOCUS       EY194903                 466 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab13e08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741481.1| PDZ domain-containing guanine
            nucleotide eXchange Factor 1 PXF-1 isoform a, PDZ-Ras-GEF factor
            (163.8 kD) (pxf-1) [Caenorhabditis elegans] gb|AAF22963.1| RA-GEF
            [Caenorhabditis elegans] emb|CAA93519.2| C. elegans PXF-1 protein
            (cor, mRNA sequence.
ACCESSION   EY194903
VERSION     EY194903.1  GI:159497749
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 845
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc gcaaacgatt ccgatgcact gctggagccg agcacaattc gcaagggcag
       61 cacgctgctg gggagccagg gcagcaggtt cggcaccagc ggaggagggg ggatgtcgcg
      121 caagaagttg tacgagcgga cgctgatgct gaagcgggtg aaggtgtatc tgcatcagat
      181 gccgatcatc gactcggagg tggagccgga ccgaatctcg ctggagtgcg aaccgcccat
      241 gaccgggggg aacagctcct ccgccgcgca gctgttccac cagcagggca gctcgacgcc
      301 ctctcggcgg cggatgccct ccccttcgcc cagcagcgtc tcctcccagt cgaaccagtc
      361 caactccgtg caccgcatcg ggctgcccaa attcggggtt gagtcgccgc aagccgtgca
      421 aaaaatgctg agtctggtgc agaactcgaa aataaagagt agagtg
//