LOCUS       EY194890                 536 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab13d02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_506352.1| Transthyretin-like family member
            precursor family member (17.8 kD) [Caenorhabditis elegans]
            pir|T20267 hypothetical protein C56A3.2 - Caenorhabditis elegans
            emb|CAB01136.1| Hypothetical protein C56A3.2 [Caenorhabditis
            elegans], mRNA sequence.
ACCESSION   EY194890
VERSION     EY194890.1  GI:159497736
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 726
            PolyA tail found inside read...SR being truncated to 686
            Potential full insert read...vec-to-vec length: 608
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt cgcttgatgt gtggtgcggc accggcgaga aatgttcgag tcaaactgtt
       61 cgatgaggat tctggacccg accctgacga ccaattagac gcaggctaca cggactcaaa
      121 tgggaacttc cagctgaagg gctccaccac cgaagcaacc aacatcgacg tcgtgttcaa
      181 gatctaccac gactgcgacg atgggattaa gcctggctct cgcaaaatca agttcaagat
      241 tcccaactcg tacgtcagtg agggagccac ccccaaaaaa acatttgaca tcggcacgct
      301 gaatctggag accatcttcg ccaacgagga gagggagttg atcgtgagca agaaaaggcg
      361 gcacttgcac cactaccggg gcggcgagaa attcaacttt ttcgttcgtc tctaggtggc
      421 aaagaaagaa gaatttggat aatggataag ccaaaacaaa caaaacaata caaatcgacc
      481 ctctttcgac ttgtcaaatt gctttttaat tgatttgcaa taaagttttt gcacca
//