LOCUS       EY194861                 255 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12h08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194861
VERSION     EY194861.1  GI:159497707
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 452
            PolyA tail found inside read...SR being truncated to 407
            Potential full insert read...vec-to-vec length: 327
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcc atccagagga ctgtggaggg gagatctcgc cttggacgaa acacgcacaa
       61 tggcaatcca tccatccaac cgaccaaaca gccactcatc gaccagtgtg ctcaatatcc
      121 cccaaacaca aaacaacgac attttgttca ctcttaacct cccaaatgtg tgttcccatt
      181 ccctctctcg cctatatatg tatttgtgta tttgggttct gtgctccgtt tcaatcaata
      241 aagttgtaaa cagca
//