LOCUS       EY194853                 278 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aab12g12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY194853
VERSION     EY194853.1  GI:159497699
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 801 was extended to value 801 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 471
            PolyA tail found inside read...SR being truncated to 429
            Potential full insert read...vec-to-vec length: 350
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcg ttttgcccaa caacaacaga cgaagacgac gaccttctcc gtgtggccca
       61 ccgacgttgt tccctctcct ccctctgccc tctccccccg ttgtccctct tttaagcaaa
      121 tgccaaggcc caccccaaac actgtctgtc tgccccgctg ttggctggcg agaatgtccg
      181 cgagaaacaa aaacttatat tctctcaata tgtgtttatt ctctctaata tccacgtccg
      241 tccatccggc taagcgaata aactcgtgtg gacacaca
//